The Effect of Insulin on Gene Expression of Inducible Nitric Oxide Synthase during Hemorrhagic Shock in Rat

The purpose of this study was to investigate the effect of insulin on gene expression of inducible nitric oxide synthase (iNOS) during hemorrhagic shock. Male Sprague-Dawley rat, weighted from 300 to 400 gram, were used for animal study model. Under anesthesia, cannulation to the femoral vessels through the dissection was done and 2 ml blood per 100 gram was withdrawn for hemorrhagic shock. After 1 hour of shock period, three groups were divided according to resuscitation fluid such as Lactated Ringer’s solution only (LR group), Lactated Ringer’s solution and 20% glucose (D20LR group), and Lactated Ringer’s solution, 20% glucose , and insulin (D20LRI group). Sampling of blood and tissue was performed after observation period for 90 minutes. The measurement of plasma nitric oxide (NO) level and iNOS gene expression in liver tissue was performed. There were no significant differences between plasma NO level of three groups. However, band density of iNOS gene expression were 18350.53 ± 6610.26 in LR group, 9148.33 ± 4081.75 in D20LR group, and 3874.07 ± 2313.40 in D20LRI group respectively with significant differences between three groups (p=0.017). This study showed insulin could suppress the gene expression of iNOS in hemorrhagic shock.